Efficient Nuclear FISH on Paraffin-Embedded Tissue Sections Using Microwave Pretreatment

Efficient nuclear FISH on paraffin-embedded tissue sections using microwave pretreatment.

Single-day FISH procedure for paraffin-embedded tissue sections using a microwave oven.

The fluorescence in situ hybridization (FISH) technique is a sensitive method for detecting gene amplification in tumor cells. However, FISH on formalin-fixed and paraffin-embedded tissue sections is time-consuming and labor-intensive (1). Tissue processing and cross-linking fixatives such as formalin may prevent the probe from accessing its target sequence, prolonging the FISH processing time ...

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

Detection of Bromodeoxyuridine in Paraffin- embedded Tissue Sections Using Microwave Antigen Retrieval is Dependent on the Mode of Tissue Fixation

bstract A simple routine microwave antigen retrieval procedure allows the sensitive detection of incorporated BrdU in pulse labeled cells. Of the two fixatives tested, Carnoy’s offers superior nuclear morphology, but with a sacrifice of immunostaining intensity. For investigations where animals are sacrificed within several hours after pulse labeling, Carnoy‘s fixative may prove adequate as a g...

Combined FISH and immunolabeling on paraffin-embedded tissue sections for the study of microchimerism.

Fluorescence in situ hybridization (FISH) has proven to be a very useful technique for the study of chimerism and microchimerism (2,4). Based on the detection of sex chromosomes, this technique allows for the identification and localization of donor and recipient cells in cases of sex-mismatched transplantation or cells from mothers and their male fetuses in studies of fetomaternal cell traffic...